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公司介绍
Polysciences公司是一家成立于1961年,致力于特殊技精细化学试剂、实验室产品,单/多聚体等产品的研发供应,所提供的产品服务于组织学(显微技术)、生物技术、电子技术、护理及制药工程等多个领域。Polysciences公司成立之初的主要产品为电镜样品制备的相关产品,目前该产品线延伸为多种组织学研究产品,包括多种方式与用途的样品包埋、优秀的染料及诊断试剂盒。同时

Polysciences/Grids - Square Mesh Grids - Standard - Gold 300mesh/100 grids/7553G-1

Polysciences/Grids - Square Mesh Grids - Standard - Gold 300mesh/100 grids/7553G-1
  • Polysciences/Grids - Square Mesh Grids - Standard - Gold 300mesh/100 grids/7553G-1
商品介绍

3.05mm diameter. Open area: 57%. Bar Width: 20µm

品牌介绍

Polysciences公司是一家成立于1961年,致力于特殊技精细化学试剂、实验室产品,单/多聚体等产品的研发供应,所提供的产品服务于组织学(显微技术)、生物技术、电子技术、护理及制药工程等多个领域。Polysciences公司成立之初的主要产品为电镜样品制备的相关产品,目前该产品线延伸为多种组织学研究产品,包括多种方式与用途的样品包埋、优秀的染料及诊断试剂盒。同时,Polysciences在对物质分离和纯化的努力中逐步发展出超顺磁性微珠,用于DNA、蛋白质、多肽的分离纯化产品,成为生命科学研究中物质分离、新蛋白(多肽)发现及功能研究的有力武器。此外,Polysciences还在高纯度单/多聚体产品领域独树一帜,通过多种技术增强单/多聚体的关键特性,并将其应用于医疗器械的研发与生产,目前Polysciences已将聚合物延伸到电子多聚体领域。公司秉承为应用而研发,目前已拥有超过3000种产品。

Polysciences, Inc. is divided into three broad divisions; Laboratory Products, Custom Synthesis & cGMP Manufacturing and Contract Manufacturing & Packaging

Laboratory Products / Catalog

This division includes thousands of products useful for histology, microscopy and life sciences applications.

Our catalog presents these products based on application areas

Life Sciences

Monomers & Polymers

Microspheres & Particles

High Performance Adhesives, Coatings & Encapsulants

Custom Synthesis & cGMP Manufacturing

Polysciences is equipped and focused on providing custom variations of products and custom designed materials to suit our customer's specialized needs.

The manufacturing facilities at Polysciences include multi-step synthesis, distillation and drying, recrystallization and filtration & clean room operations working within FDA/GMP guidelines. Learn More >

Contract Manufacturing & Formulation

Our collection of small- to mid-scale manufacturing processing and packaging equipment allows us to handle a broad array of package types including a wide variety of glass or plastic bottles, custom containers, tubes, towelettes or flame-sealed ampoules.

We have expertise in manufacturing and filling powdered products and mid-range viscosity materials into tubes. We can make, package, warehouse and ship your scientific or cosmetic product, freeing you to sell your products.



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细胞种类 中文名称 PEI 转染效率

HEK293 人胚肾细胞 90%-95%

293-T 人胚肾细胞 90%-95%

CHO-K1 仓鼠卵巢细胞 80%-90%

U251 人源神经胶质瘤细胞 80%-90%

Hela 人源宫颈癌细胞 70%-80%

COS7 猴 SV40 转化肾细胞 70%-80%

HepG2 人源肝癌细胞 70%-80%

NIH/3T3 小鼠胚胎成纤维细胞 60%-70%

胶质瘤干细胞 人源胶质瘤干细胞 60%-70%

Patu8988 人源胰腺癌细胞 60%-70%

U2OS 人源骨肉瘤细胞 60%-70%

转染试剂和 DNA 配比数据

 

细胞型号 培养基 每孔细胞数 DNA 的量 转染试剂量 和培养基混合 4-6h

293H DMEM 3×10

4

0.2µg 0.5µL DMEM+10%FBS

293FT DMEM 3×10

4

0.2µg 0.5µL DMEM+10%FBS

293E DMEM 3×10

4

0.2µg 0.5µL DMEM+10%FBS

293F DMEM 3×10

4

0.2µg 0.5µL DMEM+10%FBS

COS7 DMEM 1.5×10

4

0.4µg 0.5µL DMEM+10%FBS

hela DMEM 2×10

4

0.3µg 0.5µL 1640+15%FBS

Caco2 MEM 3.5×10

4

0.3µg 0.75µL MEM+10%FBS

BHK21 MEM 2×10

4

0.2µg 0.5µL MEM+10%FBS

CHO-DG44 DMEM+HT+pro 2×10

4

0.5µg 0.5µL DMEM+HT+pro +10%FBS

RAW264.7 DMEM 3×10

4

0.2µg 0.5µL DMEM +10%FBS

MCF7

MEM/NEAA+0.01mg/mL insulin + sodium

pyruvat

2×10

4

0.1µg 0.25µL

MEM/NEAA+0.01mg/mL insulin + sodium

pyruvat+10%FBS

SW480 IMDM 3×10

4

0.4µg 0.5µL IMDM +10%FBS

MDCK DMEM 4×10

4

0.6µg 1µL DMEM+10%FBS

CHO-K1 IMDM+Pro 3×10

4

0.2µg 0.5µL IMDM+Pro +10%FBS

HepG2 DMEM 3×10

4

0.5µg 0.75µL DMEM+10%FBS

A549 DMEM 2×10

4

0.3µg 0.5µL DMEM+10%FBS

NIH/3T3 DMEM 1.5×10

4

0.1µg 0.75µL DMEM+10%FBS

vero DMEM 3×10

4

0.3µg 0.75µL DMEM+10%FBS

sf9 SIM SF 5×10

4

0.4µg 0.75µL SIM SF+10%FBS

 

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